Development and Evaluation of a qPCR Assay for Plasmid Copy Number Determination
Program: Applied Biotechnology Master's Degree — Research and Development
Host Company: Forge Biologics
Location: Grove City, Ohio (onsite)
Student: Joseph Hill
The objective was to develop a method to measure plasmid copy number (PCN). I developed a functional proof-of-concept qPCR-based assay for PCN determination. Then, I performed initial evaluation for the PCN of samples at Forge Biologics. Finally, I explored the variability of the DNA extraction step in the workflow.