Effects of Ligand Density & Coupling Temperature on the Stability & Biotin Interference of Streptavidin-Coated Paramagnetic Particles

This project investigated how ligand density and coupling temperature affect the performance and stability of streptavidin-coated paramagnetic particles used in immunoassays. Specifically, the objective was to determine how varying the biotin-to-streptavidin molar ratio and coupling conditions influence signal generation, long-term stability, and susceptibility to interference from free biotin. Using a model estradiol (E2) competitive immunoassay on an automated chemiluminescent platform, the study evaluated changes in relative luminescent signal under different experimental conditions to identify strategies for improving assay robustness and reliability.