Evaluating the Feasibility of the Ambr250 Perfusion System as a Development Tool for N-1 Processes

This study established and utilized the Ambr250 perfusion system for the first time to model N-1 perfusion processes using Chinese Hamster Ovary (CHO) cells. N-1 perfusion is critical for achieving high cell densities and optimizing productivity in subsequent stages of upstream biologics process development. We conducted a series of experiments to evaluate key performance indicators, including cell growth, viability, gassing and metabolic activity. Additionally, we compared the results from the Ambr250 system with those obtained from larger scale 5L bioreactors to assess its reliability and accuracy as a development platform. Leveraging the automated Ambr250 system in place of traditional 5L bioreactors can significantly reduced both time and resource requirements during process development.