Optimizing a Competitive Enzyme-Linked Immunosorbent Assay (ELISA) for Enhanced Detection of Saxitoxin (STX)

For my capstone project, I worked on optimizing a competitive ELISA assay for the detection of saxitoxin (STX), a marine toxin responsible for paralytic shellfish poisoning. The main objective was to improve the assay’s sensitivity, particularly at low STX concentrations, to ensure accurate and reliable detection across a wider range of samples. My project involved testing and refining various assay conditions, such as incubation times, reagent concentrations, and buffer pH, to enhance the assay’s overall performance.