Capstone Projects

Systematic Validation and Optimization of Legacy Chromatographic Methods for Mass Spectrometer Platform Transfer

Program: Applied Biotechnology Master's — Research and Development
Host Company: LabCorp Madison
Location: Madison, Wisconsin (onsite)
Student: Benjamin Robert Flinchem

Perform cross-validation and comparative analysis on legacy chromatographic methods with the intent to transfer the method from outdated mass spectrometer hardware to newer models. Optimize methods for maximum signal sensitivity utilizing post-column infusion. Compare the results of hand-extracted validation batches ran on both the old and new mass spectrometer hardware. Critically analyze trends in parameters such as sensitivity, signal-to-noise ratio, and linearity. Use results to determine if given assays will benefit from platform transfer, while establishing a repeatable process framework for future platform transfer experiments.